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    Their rostrum and cranium are covered with tens of thousands of sensory receptors for locating swarms of zooplankton, which is their primary food source. The extraction recoveries during the preparation of the samples were found to be greater than 80% for all steroids investigated (data not shown). Evaluation of the precision revealed that both the intra- and interassay CVs were less than 16% for all analytes both when determined using a low and a high level human control sample (Table 1) and when determined using a female and a male rat control sample ().The diagnosis of male hypogonadism requires the demonstration of a low serum testosterone (T) level.We examined serum T levels in pedigreed samples taken from 62 eugonadal and 60 hypogonadal males by four commonly used automated immunoassay instruments (Roche Elecsys, Bayer Centaur, Ortho Vitros ECi and DPC Immulite 2000) and two manual immunoassay methods (DPC-RIA, a coated tube commercial kit, and HUMC-RIA, a research laboratory assay) and compared results with measurements performed by liquid chromatography-tandem mass spectrometry (LC-MSMS).

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